Delivery of nucleic acids into animal tissues by electroporation is an appealing approach for various types of gene therapy, but efficiency of existing methodsis not satisfactory. Here we present the ...

To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

Jackson Laboratory researchers have shown that using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and ...

Electroporation — the use of high-voltage electric pulses to introduce DNA/RNA/proteins into cells — has made cellular engineering and gene expression easier than ever imagined. One version of this ...

It is well understood that external electric fields can affect the integrity of cell membranes. This is due to an inherent difference between the electric potentials of the interior of a cell membrane ...

3-dimensional rendering of a cell passing through the electroporation zone while a pulse is being applied. The electric field lines which normally do not penetrate an insulating cell are able to ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

Recognized as the leader in nucleic acid delivery technologies, Mirus Bio announces the first instrument added to their product portfolio, the Ingenio® EZporator® Electroporation System. Recognized as ...

Using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and significantly improves the system's throughput over the ...

Graduate students Mingde ‘Jack’ Zheng and Joseph Sherba have developed a novel, microfluidic platform for monitoring electroporation and molecular delivery at the single cell-level as part of a NSF ...